Application

This Anti-GST Omega 1 (GSTO1), clone 1H10.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of GST Omega 1 (GSTO1).

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected GST Omega 1 (GSTO1) in human cerebral cortex and rat cerebellum tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GST Omega 1 (GSTO1), also known as Glutathione S-transferase omega-1,GSTO-1, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroascorbate reductase, Monomethylarsonic acid reductase, MMA(V), or S-(Phenacyl)glutathione reductase, SPG-R, and encoded by the gene GSTO1/GSTTLP28, a ubiquitously expressed cytoplasmic enzyme that is highest in the liver and lowest in the brain It belongs to the GST superfamily of anti-oxidant reducing enzymes and is in the omega class. The omega class of glutathione transferases (GSTs) is a relatively ancient member of the cytosolic GST superfamily, and the omega-class GSTs are found in plants, animals, and some microbial species. The omega-class, unlike other GSTs, has a cysteine residue in their active site. Consequently, the omega-class GSTs catalyze a range of thiol transferase and reduction reactions that are not catalyzed by members of the other classes. GST Omega 1 (GSTO1) is involved in L-ascorbic acid metabolism, detoxification of arsenic substances, glutathione biosynthetic processes, regulation of ryanodine-sensitive calcium release channels, regulation of skeletal muscle contraction and the release of sequestered calcium in muscle, and xenobiotic catabolism. Interestingly genetic linkage studies have discovered associations between GSTO1 gene mutations and the age at onset of Alzheimer′s disease, Parkinson′s disease, and amyotrophic lateral sclerosis, perhaps stemming from the capacity of omega-class GSTs to mitigate oxidative stress or their role in activating the proinflammatory cytokine, interleukin-1β.

Immunogen

GST-tagged recombinant protein corresponding to human GST Omega 1 (GSTO1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GST Omega 1 (GSTO1) in 200 µg of human heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~28 kDa observed